The microbiome of head and neck squamous cell carcinoma patients in Hong Kong
Refereed conference paper presented and published in conference proceedings

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AbstractRationale: The microbiota has been shown to be related to a variety of carcinomas. However, there has not been an extensive evaluation of the microbiota and its role in head and neck squamous cell carcinoma. Here we evaluate the microbial dynamics associated with HNSCC. Materials and methods: Prospectively collected HNSCC patient tumor tissues and paired normal control tissues were analyzed with NGS 16S rRNA V1-V2 region for bacteria. Bioinformatic and statistical analysis was performed with R. Results: Forty-four HNSCC patients with paired tumor and adjacent normal tissues >5cm from the tumor were evaluated. The bacterial diversity was significantly depressed in the tumor tissues when compared with that of normal controls (p<0.004). The overall oral bacterial community was commonly represented by Fusobacterium (mean abundance of 9.6%) and Prevotella (9.2%), with Streptococcus, Haemophilus and Leptotrichia constituting 5.9%, 5.6% and 5.3%, respectively. A linear discriminant analysis (LDA) for effect size (LEfSe) determined 18 “core” bacterial taxa (>1% mean abundance) were tissue-type discriminative. Among them, Fusobacterium was significantly predominant in tumor tissues when compared with normal controls (mean abundance of 12.9% vs 6.3%, p<0.001). Other dominant bacteria in tumor tissues include Peptostreptococcus, Parvimonas, Catonella, Johnsonella, Peptostreptococcaceae (family) and Staphylococcus. In contrast, Streptococcus and another 10 genera were more common in normal tissues. Conclusions: Our early results show that there is a reduced diversity of the microbiome and an increased abundance of fusobacterium in HNSCC. However, the species and relationship with the pathogenesis of HNSCC still needs to be investigated as these findings may provide essential information for further preventative or management strategies to control HNSCC.
All Author(s) ListJason YK Chan, Cherrie WK Ng, Paul KS Chan, Zenon WC Yeung, Eddy WY Wong, AC Vlantis, Zigui Chen
Name of Conference8th European Congress on Head and Neck Oncology
Start Date of Conference11/04/2018
End Date of Conference14/04/2018
Place of ConferenceRome
Country/Region of ConferenceItaly
LanguagesEnglish-United Kingdom

Last updated on 2018-18-05 at 16:09