Utilizing digital methylation PCR for detection of head and neck squamous cell carcinoma
Refereed conference paper presented and published in conference proceedings


摘要Rationale: Promoter methylation of the tumour suppressor genes has been previously observed in the various type of cancers, including Head and Neck Squamous Cell Carcinoma(HNSCC). It is hoped that using the detection of the methylation signature could act as the marker for early detection and diagnosis of HNSCC, which will allow identification of patients at an earlier stage of the disease. Here we evaluate the use digital droplet PCR detection of methylation markers in the early detection of HNSCC. Materials and methods: Tumour and paired normal tissues were prospectively collected from eligible HNSCC patients, also along with the collection of tissues from non-cancer control subjects. Promoter methylation of reported tumour suppressor genes in HNSCC patient tumour tissues, HNSCC oral rinses, normal control tissues and normal oral rinses were assessed using digital droplet PCR. The data was analysed by Mann-Whitney U test, Student’s t-test comparison of means and ROC analysis of the samples for sensitivity and specificity with MedCalc Statistical Software version 17.6 (MedCalc Software bvba, Ostend, Belgium; http://www.medcalc.org; 2017). Results: Samples from 55 HNSCC patients and 26 non-cancer subjects were analysed. (Patient age range: 31 yr-92 yr, median: 66 yr; Gender: 39 M, 16 F). Using digital droplet PCR there was significant difference in the methylation density of PAX5 (P<0.001), EDNRB (P<0.001), DCC (P<0.001), MGMT (P<0.007), DAPK (P<0.03) and P16 (P = 0.03) when comparing HNSCC with paired normal tissues. PAX5 (P<0.001), EDNRB (P<0.001), DCC (P<0.001) shown aberrant methylation when compared with control tissues. A further analysis of oral rinses between HNSCC and control patients for PAX5 showed a sensitivity of 89.1% and specificity of 73.1% (PPV: 87.5%, NPV: 76%). EDNRB demonstrated a lower sensitivity of 46.4% and specificity of 92.3% (PPV: 89.7%, NPV: 44.2%) for oral rinses when compared between HNSCC and control patients. Conclusions: Using digital methylation PCR may provide a sensitive method for the detection of HNSCC, with the usage of the PAX5 methylation detection in oral rinse setting could provide a relative less invasive method for the detection of HNSCC.
著者Sherwood YH Fung, Jason YK Chan, Cherrie WK Ng, Avis WI Shiu, Allen KC Chan
會議名稱8th European Congress on Head and Neck Oncology

上次更新時間 2018-18-05 於 16:03