Activation of Eosinophils Interacting with Bronchial Epithelial Cells by Antimicrobial Peptide LL-37 in Allergic Asthma
Other conference paper



The role of antimicrobial peptide LL-37 in asthma exacerbation is unclear, though microbial infections,
which is the most common inducer of asthma exacerbation, is accompanied by elevated LL-37 in innate
immune cells.
The present study is to evaluate the immunopathological roles of LL-37 in allergic asthma.
The present study found that co-culture of eosinophils and bronchial epithelial cell line BEAS-2B
significantly enhanced intercellular adhesion molecule-1 on both cells and CD18 expression on
eosinophils upon LL-37 stimulation. Inflammatory IL-6, CXCL8 and CCL4 were substantially released
in co-culture in the presence of LL-37. LL-37 triggered the activation of eosinophils interacting with
BEAS-2B cells in a P2X purinoceptor 7/epidermal growth factor receptor-dependent manner.
Eosinophils and BEAS-2B cells differentially contributed to the expression of cytokines/chemokines in
co-culture, while soluble mediators were sufficient to mediate the intercellular interactions. Intracellular
p38-mitogen-activated protein kinase, extracellular signal-regulated kinase and NF-kB signaling
pathways were essential for LL-37-mediated activation of eosinophils and BEAS-2B cells. By using the
ovalbumin-induced asthmatic model, we observed that the intranasal administration of mCRAMP
(mouse ortholog of LL-37) in combination with ovalbumin during the allergen challenge stage
significantly enhanced airway hyper-responsiveness and airway inflammation in sensitized mice.
Results therefore implicated a deteriorating and inflammatory role of LL-37 in allergic asthma. This
study provides evidence of LL-37 in triggering asthma exacerbation via the activation of eosinophils
interacting with bronchial epithelial cells in the inflammatory airway.
著者Chun-Kwok Wong, Delong Jiao, Miranda Sin-Man Tsang, Jing Zhu, Man Chu, Dehua Liu, Christopher Wai-Kei Lam
會議名稱European Academy of Allergy and Clinical Immunology Congress 2017
會議地點Helsinki Messukeskus

上次更新時間 2018-13-06 於 10:02