Noninvasive reconstruction of placental methylome from maternal plasma DNA: potential for prenatal testing and monitoring
Publication in refereed journal

替代計量分析
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其它資訊
摘要Objective:
During human pregnancy, the DNA methylation of placental tissue is highly relevant to the normal growth and development of the fetus; therefore, methylomic analysis of the placental tissue possesses high research and clinical value in prenatal testing and monitoring. Thus, our aim is to develop an approach for reconstruction of the placental methylome, which should be completely noninvasive and achieve high accuracy and resolution.
Results:
We propose a novel size‐based algorithm, FEtal MEthylome Reconstructor (FEMER), to noninvasively reconstruct the placental methylome by genomewide bisulfite sequencing and size‐based analysis of maternal plasma DNA. By applying FEMER on a real clinical dataset, we demonstrate that FEMER achieves both high accuracy and resolution, thus provides a high‐quality view of the placental methylome from maternal plasma DNA. FEtal MEthylome Reconstructor could also predict the DNA methylation profile of CpG islands with high accuracy, thus shows potential in monitoring of key genes involved in placental/fetal development. Source code and testing datasets for FEMER are available at http://sunlab.cpy.cuhk.edu.hk/FEMER/.
Conclusion:
FEtal MEthylome Reconstructor could enhance the noninvasive fetal/placental methylomic analysis and facilitate its application in prenatal testing and monitoring.
出版社接受日期05.01.2018
著者Kun Sun, Fiona M.F. Lun, Tak Y. Leung, Rossa W.K. Chiu, Y.M. Dennis Lo, Hao Sun
期刊名稱Prenatal Diagnosis
出版年份2018
月份2
卷號38
期次3
頁次196 - 203
國際標準期刊號0197-3851
語言英式英語

上次更新時間 2020-06-07 於 00:14