Regulation of Host Genome Methylation and Expression in HIV Infection
Refereed conference paper presented and published in conference proceedings

香港中文大學研究人員

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摘要It has been shown that both the methylation and gene expression play roles in HIV-host interplay. In this study, to further investigate the relationship between HIV infection and patterns of genome-wide methylation and gene expression, we performed MeDIP-seq and RNA-seq for five T cell lines and each cell line contained HIV+ and control samples. Within differentially expressed genes (DEGs) between HIV+ and control samples, the top enriched pathways included “primary immunodeficiency” and “inflammatory response”. Eighteen up-regulated and eight down-regulated DEGs were shared by four cell lines. Moreover, on average, 1,736 differentially methylated regions (DMRs) were found between HIV+ and control samples for each cell line. DMRs were significantly enriched in promoter and exon regions. The top enriched pathways of DMR-associated genes were “ubiquitin-dependent protein catabolic process” and “GTPase activator activity”. Besides, 635 genes were also involved in the crosstalk of methylation and expression, out of which ATP1B1, CAMK2D, GRIN2A, MAPK10, CACNA1C and F2R were shared by more than two cell lines and enriched in “cAMP signaling pathway”, which affects HIV replication and infection. This study broadens our understanding on the mechanism of HIV/AIDS and provides a resource about dynamic changes at the transcriptome and epigenetic levels during HIV infection.
著者ZENG Xi, TSUI Kwok Wing Stephen
會議名稱2018 8th International Conference on Bioscience, Biochemistry and Bioinformatics (ICBBB 2018)
會議開始日18.01.2018
會議完結日20.01.2018
會議地點Tokyo
會議國家/地區日本
出版年份2018
月份1
日期18
語言美式英語
關鍵詞Gene expression, HIV infection, methylation, transcriptome

上次更新時間 2018-30-11 於 10:27