Profiling DNA Methylation Using Bisulfite Sequencing (BS-Seq)
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AbstractDNA cytosine methylation is one of the most abundant epigenetic marks found in the plant nuclear genome. Bisulfite sequencing (BS-Seq) is the method of choice for profiling DNA cytosine methylation genome-wide at a single nucleotide resolution. The basis of this technique is that the unmethylated cytosine can be deaminated to uracil by sodium bisulfite, while the methylated cytosine is resistant to the treatment. By deep sequencing of the bisulfite converted genomic DNA, the methylation level of each mappable cytosine position in the genome could be measured. In this chapter, we present a detailed 2-day protocol for performing a BS-Seq experiment and a simple bioinformatic workflow for wet lab biologists to visualize the methylation data.
All Author(s) ListYun-Ru Chen, Sheng Yu, Silin Zhong
All Editor(s) ListMarian Bemer, Célia Baroux
Journal nameMethods in molecular biology (Clifton, N.J.)
Book titlePlant Chromatin Dynamics: Methods and Protocols
Series TitleMethods in Molecular Biology
Volume Number1675
PublisherHumana Press, New York, NY
Place of PublicationNew York
Pages31 - 43
LanguagesEnglish-United Kingdom

Last updated on 2021-07-05 at 00:21