Structural Characterization of Intact Glycoconjugates by Tandem Mass Spectrometry Using Electron-Induced Dissociation
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要Characterizing the structures of glycoconjungates is important because of glycan heterogeneity and structural complexity of aglycon. The presence of relatively weak glycosidic linkages leads to preferential cleavages that limit the acquisition of structural information under typical mass spectrometry dissociation conditions, such as collision-induced dissociation (CID) and infrared multiphoton dissociation. In this paper, we explored the dissociation behaviors of different members of glycoconjugates, including glycopeptides, glycoalkaloids, and glycolipids, under electron-induced dissociation (EID) conditions. Using CID spectra as references, we found that EID is not only a complementary method to CID, but also a method that can generate extensive fragment ions for the structural characterization of all intact glycoconjugates studied. Furthermore, isomeric ganglioside species can be differentiated, and the double bond location in the ceramide moiety of the gangliosides can be identified through the MS3 approach involving sequential CID and EID processes.
著者Y. L. Elaine Wong, Xiangfeng Chen , Ri Wu, Y. L. Winnie Hung, T.-W. Dominic Chan
期刊名稱Analytical Chemistry
出版年份2017
月份9
日期19
卷號89
期次18
出版社ACS Publication
出版地USA
頁次10111 - 10117
國際標準期刊號0003-2700
電子國際標準期刊號1520-6882
語言美式英語
關鍵詞Glycoconjugates, Tandem Mass Spectrometry, Electron-Induced Dissociation

上次更新時間 2021-19-01 於 01:31