Generation and Characterization of Gas-Phase Doubly Charged Biradical Peptide Ions (M2+••)
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摘要The gas-phase chemistry of peptide radical ions is attracting considerable interest in the fields of biology and mass spectrometry owing to its capability to provide sequence information on peptides and proteins. In this study, we observed that doubly charged peptide ions (M2+) can be produced from the collision-induced dissociation (CID) of Hg(II)-adducted peptide ions. The chemical nature and, thus, the dissociation pathways of this hydrogen-deficient biradical M2+ species is intriguing. We investigated the generation and dissociation behavior of this M2+ species under electron-capture dissociation (ECD) and CID conditions. The side-chain loss in the CID of the charge-reduced M+• ions formed by single-electron capture suggested that M2+ existed as a biradical ion. This ion underwent the combination of the two radical sites and conversion to hydrogen surplus species through structural rearrangement with increased energies. This study demonstrated a promising method to generate reactive doubly charged biradical precursor ions and, thus, help characterize novel biomolecules.
著者Y. L. Elaine Wong, Xiangfeng Chen , Ri Wu, Y. L. Winnie Hung, Hoi Sze Yeung, T.-W. Dominic Chan
期刊名稱Analytical Chemistry
出版年份2017
月份7
日期18
卷號89
期次14
出版社ACS publication
出版地USA
頁次7773 - 7780
國際標準期刊號0003-2700
電子國際標準期刊號1520-6882
語言美式英語
關鍵詞Biradical Peptide Ions, Mass Spectrometry, ECD, CID

上次更新時間 2021-22-02 於 00:42