From Systematic Review to the Development of a Clinically Relevant Osteoporotic Metaphyseal Fracture Animal Model for Bone Healing Enhancement Mechanistic Studies
Refereed conference paper presented and published in conference proceedings


摘要Introduction: Most osteoporotic fractures occur at the metaphyseal bone region but few models exist and the healing is poorly understood. This study aimed: (1) to conduct a systematic review to analyse current osteoporotic metaphyseal fracture animal models and (2) to create a new osteoporotic metaphyseal fracture model.

Methods: Literature search was performed on the PubMed, Embase and Web of Science databases and relevant articles were reviewed. For the creation of the model, 26 6-month-old Sprague-Dawley rats were obtained. All rats underwent induction of osteoporosis. A 6-hole T-shaped mini-plate and screws were used to fix the plate to the distal femur and an osteotomy was performed. X-ray of the femur was taken weekly and the rats were euthanised at 1, 2, 4, and 6 weeks postsurgery. For analysis, sections cut longitudinally were stained with haematoxylin and eosin.

Results: A total of 19 studies were included in the systematic review. We proposed that an animal model should satisfy the following criteria: (1) induction of osteoporosis, (2) unilateral complete osteotomy or defect at the metaphysis, and (3) internal fixation. There was complete bony bridging in all animals at 6 weeks. Intramembranous ossification was observed during the healing phase.

Discussion and Conclusion: Upon detailed analysis of the current models we have derived a recommendation and created a valid and reproducible metaphyseal fracture model that satisfies these criteria, which can provide a platform for future quality studies to enhance osteoporotic fracture healing.

Acknowledgement: Health and Medical Research Fund (Ref: 04152406)
著者Wong Ronald Man Yeung, Choy Man Huen Victoria, Leung Kwok Sui, Chow Simon Kwoon Ho, Cheung Wing Hoi, Cheng Jack Chun Yiu
會議名稱Hong Kong Orthopaedic Association 37th Annual Congress 2017
會議地點Hong Kong
會議論文集題名The Proceedings of the Hong Kong Orthopaedic Association 37th Annual Congress 2017
出版地Hong Kong
頁次22 - 22

上次更新時間 2019-03-12 於 14:28