Synaptic connectivity between neurons in the lateral geniculate nucleus and primary visual cortex by viral tools
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AbstractActivities from randomly sampled neurons in chosen pre- and post- synaptic CNS regions are often used in scientific studies to evaluate complex cross-region pathways such as thalamo-cortical pathways. These sampled activities are from neurons that may not necessarily have actual connections with each other. Combining current generation of viral techniques, the use of vesicular stomatitis virus (VSV) and the single cell electroporation procedure, selectively-targeted single-cell retrograde monosynaptic labelling can be achieved and provide more accurate and representative results. We applied these tools to re-evaluate certain aspects of the visual thalamo-cortical pathway. Custom-designed stimuli were presented to mice in vivo. Real-time fluorescence signals from calcium indicator GCaMP6f were captured under a two photon microscope setup to identify simple cells in the primary visual cortex (V1). Subsequent electroporation of retrograde glycoprotein RABV-G and TVA receptor encoding transgenes into the chosen cell(s) allow specific VSV infection. Existing studies that used locally injected viral or fluorescent labelling discovered that the V1 receives monocular input from the dorsal lateral geniculate nucleus (dorsal LGN). Revealing upstream neurons to an identified V1 simple cell provides detailed information with more specificity about its presynaptic LGN population density and location, and enables further functional studies on the LGN-to-V1 thalamocortical network.
All Author(s) ListH. CHAN, D.C.W. CHAN, X.F. YANG, Y. KE, W.H. YUNG
Name of ConferenceNeuroscience 2017 (Society for Neuroscience Annual Meeting 2017)
Start Date of Conference11/11/2017
End Date of Conference15/11/2017
Place of ConferenceWashington DC
Country/Region of ConferenceUnited States of America
LanguagesEnglish-United Kingdom

Last updated on 2018-30-04 at 12:09