Effects of neonatal maternal separation on the colonic motility and enteric nervous system of adult rats
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摘要Background:
Irritable bowel syndrome (IBS) is a common functional bowel disorder with recurrent abdominal pain and altered bowel movements, but its causes have not been fully understood. Adult rats after neonatal maternal separation show visceral hypersensitivity and increased colonic motility, and hence have been used as an animal model of human IBS.

Objective:
The objective of this study was to investigate the effects of neonatal maternal separation on both colonic motility of adult rats in vivo and colonic muscle contractility of rat colon explants ex vivo, and to determine the changes of the enteric nervous system (ENS) which comprises the intrinsic innervation of the gastrointestinal tract and controls the colonic motility.

Patients and Methods / Material and Methods:
Neonatal rats of the maternal-separated group (MS) were separated from their mothers for 3 hours daily from Postnatal day P2 to P22 while non-handled (NH) control rats were kept with their mothers. All rats were weaned at P23. The colonic motility was studied in vivo and ex vivo while the changes of the ENS was studied by immunohistochemistry.

Results:
The electrical field stimulation-induced longitudinal muscle contraction of the MS adult rat colon in the organ bath was found to have increased as compared to that of the NH colon. Immunostaining results indicated that the proportion of nNOS immunoreactive myenteric neurons was decreased.

Conclusion:
Neonatal maternal separation could increase the colonic motility of adult rats both in vivo and ex vivo, and these increases were associated with the changes of the enteric nervous system.
著者Bo LI, Cynthia Kwan Yui CHEUNG, John Anthony RUDD, Justin Che Yuen WU, Wood Yee CHAN
會議名稱XXIII World Congress of Neurology
會議開始日16.09.2017
會議完結日21.09.2017
會議地點Kyoto
會議國家/地區日本
出版年份2017
語言英式英語
關鍵詞Maternal separation, Colonic motility, Enteric nervous system, Electrical field stimulation, Adult rats

上次更新時間 2018-27-04 於 15:38