Investigation of CRL3 E3 Ligases in Plant Lateral Organ Development
Other conference paper


摘要Transcription factors (TFs) play crucial roles in regulating transcriptional activities, plants development and metabolism. For plants, they are also key components for regulatory networks in controlling responses to biotic and abiotic stresses. In sessile plants, a large number of proteins that include conserved MYB DNA-binding domain, MYB TFs, are mainly expressing in diverse plant organs and participating in plant developmental events. Thus, the post-translational regulation on the activities of these TFs is a dynamic mechanism for plants. Because of the important and versatile functions, the ubiquitin proteasome pathway has a central role to equilibrate the protein levels in planta. As the key regulators within this pathway, E3 ubiquitin ligases could efficiently target substrate proteins and promote proteasomal degradation. As both E3 ubiquitin ligases and MYB TFs are essential components for plants development, here we propose to investigate the regulatory relationship between them. We hypothesize that the E3 ligases could assemble with MYB TFs as substrates for the proteasomal degradation and modulate their transcriptional activities. To test this hypothesis, we investigate whether substrate adaptors CULLIN3-RING ubiquitin ligases (CRL3s) have the physical interaction with lateral organ expressed MYB TFs. Thereafter we aim to determine whether CRL3 assembled MYBs would be degraded via proteasome dependent pathway. Our study will discover the wide spectrum of CRL3 assembled substrates. Noteworthy, these investigations shall establish the functional connection between the CRL3 E3 Ligases and plant lateral organ development. Supported by General Research Fund from the Research Grants Council of Hong Kong (GRF/CUHK14104716) to L.C.
著者Liu HONG, Liwen JIANG, Liyuan CHEN
會議名稱The Cold Spring Harbor Asia conference on Plant Cell & Development Biology

上次更新時間 2018-16-04 於 18:25