Arabidopsis COG Complex Subunits COG3 and COG8 Modulate Golgi Morphology, Vesicle Trafficking Homeostasis and Are Essential for Pollen Tube Growth
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摘要Spatially and temporally regulated membrane trafficking events incorporate membrane and cell wall materials into the pollen tube apex and are believed to underlie the rapid pollen tube growth. In plants, the molecular mechanisms and physiological functions of intra-Golgi transport and Golgi integrity maintenance remain largely unclear. The conserved oligomeric Golgi (COG) complex has been implicated in tethering of retrograde intra-Golgi vesicles in yeast and mammalian cells. Using genetic and cytologic approaches, we demonstrate that T-DNA insertions in Arabidopsis COG complex subunits, COG3 and COG8, cause an absolute, male-specific transmission defect that can be complemented by expression of COG3 and COG8 from the LAT52 pollen promoter, respectively. No obvious abnormalities in the microgametogenesis of the two mutants are observed, but in vitro and in vivo pollen tube growth are defective. COG3 or COG8 proteins fused to green fluorescent protein (GFP) label the Golgi apparatus. In pollen of both mutants, Golgi bodies exhibit altered morphology. Moreover, gamma-COP and EMP12 proteins lose their tight association with the Golgi. These defects lead to the incorrect deposition of cell wall components and proteins during pollen tube growth. COG3 and COG8 interact directly with each other, and a structural model of the Arabidopsis COG complex is proposed. We believe that the COG complex helps to modulate Golgi morphology and vesicle trafficking homeostasis during pollen tube tip growth.
著者Tan XY, Cao K, Liu F, Li YX, Li PX, Gao CJ, Ding Y, Lan ZY, Shi ZX, Rui QC, Feng YH, Liu YL, Zhao YX, Wu CY, Zhang Q, Li Y, Jiang LW, Bao YQ
期刊名稱PLOS GENETICS
出版年份2016
月份7
日期22
卷號12
期次7
出版社PUBLIC LIBRARY SCIENCE
頁次e1006140
國際標準期刊號1553-7390
語言英式英語
Web of Science 學科類別Genetics & Heredity;Genetics & Heredity

上次更新時間 2021-15-06 於 00:13