A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation
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AbstractPost-Golgi protein sorting and trafficking to the plasma membrane (PM) is generally believed to occur via the trans-Golgi network (TGN). In this study using Nicotiana tabacum pectin methylesterase (NtPPME1) as a marker, we have identified a TGN-independent polar exocytosis pathway that mediates cell wall formation during cell expansion and cytokinesis. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that Golgi-derived secretory vesicles (GDSVs) labeled by NtPPME1-GFP are distinct from those organelles belonging to the conventional post-Golgi exocytosis pathway. In addition, pharmaceutical treatments, superresolution imaging, and dynamic studies suggest that NtPPME1 follows a polar exocytic process from Golgi-GDSV-PM/cell plate (CP), which is distinct from the conventional Golgi-TGN-PM/ CP secretion pathway. Further studies show that ROP1 regulates this specific polar exocytic pathway. Taken together, we have demonstrated an alternative TGN-independent Golgi-to-PM polar exocytic route, which mediates secretion of NtPPME1 for cell wall formation during cell expansion and cytokinesis and is ROP1-dependent.
All Author(s) ListWang H, Zhuang XH, Wang XF, Law AHY, Zhao T, Du SW, Loy MMT, Jiang LW
Journal namePlant Physiology
Volume Number172
Issue Number2
PublisherAmerican Society of Plant Biologists
Pages1003 - 1018
LanguagesEnglish-United Kingdom
Web of Science Subject CategoriesPlant Sciences;Plant Sciences

Last updated on 2020-04-08 at 03:22