MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development
Publication in refereed journal

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其它資訊
摘要Programmed cell death (PCD)-triggered degradation of plant tapetum is essential for microspore development and pollen coat formation; however, little is known about the cellular mechanism regulating tapetal PCD. Here, we demonstrate that Rab7-mediated vacuolar transport of tapetum degradation-related cysteine proteases is crucial for tapetal PCD and pollen development in Arabidopsis (Arabidopsis thaliana), with the following evidence: (1) The monensin sensitivity1 (mon1) mutants, which are defective in Rab7 activation, showed impaired male fertility due to a combined defect in both tapetum and male gametophyte development. (2) In anthers, MON1 showed preferential high level expression in tapetal cell layers and pollen. (3) The mon1 mutants exhibited delayed tapetum degeneration and tapetal PCD, resulting in abnormal pollen coat formation and decreased male fertility. (4) MON1/ CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-mediated Rab7 activation was indispensable for vacuolar trafficking of tapetum degradation-related cysteine proteases, supporting that PCD-triggered tapetum degeneration requires Rab7-mediated vacuolar trafficking of these cysteine proteases. (5) MON1 mutations also resulted in defective pollen germination and tube growth. Taken together, tapetal PCD and pollen development require successful MON1/ CCZ1-mediated vacuolar transport in Arabidopsis.
著者Yong Cui, Qiong Zhao, Hong-Tao Xie, Wing Shing Wong, Xiangfeng Wang, Caiji Gao, Yu Ding, Yuqi Tan, Takashi Ueda, Yan Zhang, Liwen Jiang
期刊名稱Plant Physiology
出版年份2017
月份1
卷號173
期次1
出版社AMER SOC PLANT BIOLOGISTS
頁次206 - 218
國際標準期刊號0032-0889
電子國際標準期刊號1532-2548
語言英式英語
Web of Science 學科類別Plant Sciences;Plant Sciences

上次更新時間 2020-20-10 於 03:44