Role of transient receptor potential vanilloid 1 channels in the regulation of functional properties of embryonic stem cell-derived cardiomyocytes
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AbstractTransient receptor potential (TRP) channels are broadly expressed in a variety of tissues and cell types and they are able to respond to a wide range of stimuli in the cellular environment. Among TRP channels, TRPV1 channel is activated by heat, pain inducers and the pungent component of hot chili peppers capsaicin. Previous studies showed that upon stimulation, TRPV1 channel can generate intracellular Ca2+ concentration ([Ca2+]i) changes by Ca2+ entry via the plasma membrane or by Ca2+ release from intracellular organelles. As a fundamental property of cardiomyocytes (CMs), [Ca2+]i plays an important role in different cellular processes such as excitation-contraction coupling, cell proliferation and cell death. However, there is only limited knowledge on the function of TRPV1 channel in the Ca2+-handling properties and electrophysiology characters of CMs. In this study, we used embryonic stem cells (ESCs)-derived CMs as a model to study the role of TRPV1 channel in CMs. Our recent results revealed that TRPV1 agonist, capsaicin decreased the rate and diastolic depolarization slope of action potential of ESC-derived CMs, as well as the amplitude and frequency of spontaneous Ca2+ transients of ESC-derived CMs. To study the role of TRPV1 in ESC-derived CMs, TRPV1 will be functionally knocked down by the dominant negative form of TRPV1, TRPV1β. Adenovirus harboring the TRPV1β will be made in the near future. This study will not only give a better understanding of Ca2+ homeostasis of ESC-derived CMs, but also provide insights into the future cell replacement therapies.
All Author(s) ListRui Zhao, Zenghua Qi, Xiaoqiang Yao, Suk Ying Tsang
Name of ConferenceHong Kong Inter-University Postgraduate Symposium in Biochemical Sciences
Start Date of Conference16/06/2017
End Date of Conference16/06/2017
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2017
LanguagesEnglish-United Kingdom

Last updated on 2018-23-01 at 02:48