Role of TRPC7 in regulating the functions of embryonic stem cell-derived cardiomyocytes
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AbstractIon channels are vital molecular device to maintain the electrophysiological function and calcium homeostasis of cardiomyocytes. Although classical sodium, potassium and calcium channels are intensively studied, many other channels that may also contribute to the subtle regulation of cardiomyocytes remain largely unexplored. Canonical Transient Receptor Potential channel (TRPC) is non-selective cation channel activated by G protein coupled receptors. TRPC channel widely expresses in different tissue, playing an important role in the maintenance of normal cell function. The aim of this project is to study the function of TRPC7 in cardiomyocytes. Western blotting showed TRPC7 is expressed in mouse heart, and detection of multiply bands indicating more than one isoforms may exist in heart. Immunocytochemistry experiments showed that TRPC7 locates at plasma membrane in early differentiation stage mouse embryonic stem cell-derived cardiomyocytes (mESC-CMs), but translocates from membrane to the sarcomere during the maturation of mESC-CMs. Translocation of TRPC7 occurs during the whole process of maturation; TRPC7 preferentially locates near M-line in intermediate differentiation stage mESC-CMs while it locates in Z-line in late differentiation stage mESC-CMs. We speculated that both full-length-TRPC7 and truncated-TRPC7 (which lacks the transmembrane pore region) may exist in cardiomyocytes. The full-length-TRPC7, with its ion channel function, may participate in the regulation of electrophysiological function of cardiomyocytes in early differentiation stage, while the truncated-TRPC7 may insert into sarcomere to facilitate the stability of sarcomere.
All Author(s) ListXian Ji Liu, Suk Ying Tsang
Name of ConferenceInter-University Postgraduate Symposium in Biochemical Sciences
Start Date of Conference16/06/2017
End Date of Conference16/06/2017
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
LanguagesEnglish-United Kingdom

Last updated on 2018-23-01 at 02:48