MyoD and FoxO3 mediated hotspot interaction orchestrates super-enhancer activity during myogenic differentiation
Invited conference paper presented and published in conference proceedings


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AbstractSuper-enhancers (SEs) are cis-regulatory elements enriching lineage specific key transcription factors (TFs) to form hotspots. A paucity of identification and functional dissection promoted us to investigate SEs during myoblast differentiation. ChIP-seq analysis of histone marks leads to the uncovering of SEs which remodel progressively during the course of differentiation. Further analyses of TF ChIP-seq enable the definition of SE hotspots co-bound by the master TF, MyoD and other TFs, among which we perform in-depth dissection for MyoD/FoxO3 interaction in driving the hotspots formation and SE activation. Furthermore, using Myogenin as a model locus, we elucidate the hierarchical and complex interactions among hotspots during the differentiation, demonstrating SE function is propelled by the physical and functional cooperation among hotspots. Lastly, we show MyoD and FoxO3 are key in orchestrating the Myogenin hotspots interaction and activation. Altogether our results identify muscle specific SEs and provide mechanistic insights into the functionality of SE.
All Author(s) ListXianlu Laura Peng, Karl Kamhei So, Liangqiang He, Yu Zhao, Jiajian Zhou, Yuying Li, Mingze Yao, Bo Xu, Suyang Zhang, Hongjie Yao, Ping Hu, Hao Sun, Huating Wang
Name of Conference2017 Annual Meeting of Chinese Society for Cell Biology
Start Date of Conference26/09/2017
End Date of Conference29/09/2017
Place of ConferenceXiamen
Country/Region of ConferenceChina
Year2017
LanguagesEnglish-United States

Last updated on 2018-23-05 at 11:28