Smad7 protects against acute kidney injury by rescuing tubular epithelial cells from the G1 cell cycle arrest
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摘要Smad7 plays a protective role in chronic kidney disease; however, its role in acute kidney injury (AKI) remains unexplored. Here, we report that Smad7 protects against AKI by rescuing the G1 cell cycle arrest of tubular epithelial cells (TECs) in ischemia/reperfusion-induced AKI in mice in which Smad7 gene is disrupted or restored locally into the kidney. In Smad7 gene knockout (KO) mice, more severe renal impairment including higher levels of serum creatinine and massive tubular necrosis was developed at 48 h after AKI. In contrast, restored renal Smad7 gene locally into the kidney of Smad7 KO mice protected against AKI by promoting TEC proliferation identified by PCNA+ and BrdU+ cells. Mechanistic studies revealed that worsen AKI in Smad7 KO mice was associated with a marked activation of TGF-β/Smad3-p21/p27 signaling and a loss of CDK2/cyclin E activities, thereby impairing TEC regeneration at the G1 cell cycle arrest. In contrast, restored Smad7 locally into the kidneys of Smad7 KO mice protected TECs from the G1 cell cycle arrest and promoted TEC G1/S transition via a CDK2/cyclin E-dependent mechanism. In conclusion, Smad7 plays a protective role in AKI. Blockade of TGF-β/Smad3-p21/p27-induced G1 cell cycle arrest may be a key mechanism by which Smad7 treatment inhibits AKI. Thus, Smad7 may be a novel therapeutic agent for AKI.
出版社接受日期13.07.2017
著者Sha Fu, Ying Tang, Xiao R. Huang, Min Feng, An P. Xu, Hui Y. Lan
期刊名稱Clinical Science
出版年份2017
月份8
卷號131
期次15
出版社Portland Press
出版地United Kingdom
頁次1955 - 1969
國際標準期刊號0143-5221
電子國際標準期刊號1470-8736
語言英式英語
關鍵詞Smad3, Smad7, acute kidney injury, cyclin dependent kinases, cycline

上次更新時間 2021-29-07 於 00:02