Optical micro-Printing of Cellular-Scale Microscaffold Arrays for 3D Cell Culture
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要Guiding cell culture via engineering extracellular microenvironment has attracted tremendous attention due to its appealing potentials in the repair, maintenance, and development of tissues or even whole organs. However, conventional biofabrication technologies are usually less productive in fabricating microscale three-dimensional (3D) constructs because of the strident requirements in processing precision and complexity. Here we present an optical micro-printing technology to rapidly fabricate 3D microscaffold arrays for 3D cell culture and cell-scaffold interaction studies on a single chip. Arrays of 3D cubic microscaffolds with cubical sizes matching the single-cell size were fabricated to facilitate cell spreading on suspended microbeams so as to expose both apical and basal cell membranes. We further showed that the increasing of the cubical size of the microscaffolds led to enhanced spreading of the seeded human mesenchymal stem cells and activation of mechanosensing signaling, thereby promoting osteogenesis. Moreover, we demonstrated that the spatially selective modification of the surfaces of suspended beams with a bioactive coating (gelatin methacrylate) via an in-situ printing process allowed tailorable cell adhesion and spreading on the 3D microscaffolds.
著者Xia Ouyang, Kunyu Zhang, Jushuai Wu, Dexter Siu-Hong Wong, Qian Feng, Liming Bian, A. Ping Zhang
期刊名稱Scientific Reports
出版年份2017
月份8
日期21
卷號7
頁次8880
國際標準期刊號2045-2322
電子國際標準期刊號2045-2322
語言美式英語

上次更新時間 2021-19-02 於 00:28