Regulation and Function of Histone Demethylase JMJD3 in HBV-induce Hepatocarcinogenesis
Refereed conference paper presented and published in conference proceedings


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AbstractIntroduction and Project Objectives: Previous studies have demonstrated that JMJD3 (a jmjd domain containing histone demethylase) played a crucial role in inflammation. We aimed to investigate the functional roles of JMJD3 in hepatocellular carcinoma (HCC). Methods: Cell proliferation, wound healing, colony formation assays were used to evaluate the roles of JMJD3 in HCC in vitro. Anchorage-independent cell growth assay and in vivo tumor xenograft mouse model were used to examine the roles of JMJD3 in HCC cell transformation and tumorigenesis. Tissue microarray, western blotting and chromatin immunoprecipitation were performed to delineate the mechanism underlying JMJD3 regulation on its downstream targets. Results: Our study showed that JMJD3 was significantly down-regulated in HCC cell lines and tissues. Restored expression of JMJD3 inhibited oncogenic phenotypes of HCC cells in vitro and tumorigenicity in vivo. These suggested a tumor suppressive role of JMJD3 in HCC. DACH1 was identified as one of the JMJD3 downstream targets. Tissue microarray analysis showed a positive correlation between the expression of JMJD3 and DACH1 in HCC. DACH1 promoter was found to be in a high H3K27 tri-methylation (H3K27me3) status which was attenuated by ectopic expression of JMJD3 in HCC cells. This result, suggested that JMJD3 regulated DACH1 expression by histone demethylation. We found that HBx suppressed JMJD3 expression through activation of miR-29a in HCC. Conclusions: JMJD3 plays tumor suppressive roles in HCC by regulating DACH1 expression.
All Author(s) ListXIAO Zhangang, TO Ka Fai, WANG Yan, CHEN Yangchao
Name of ConferenceHealth Research Symposium 2017
Start Date of Conference16/06/2017
End Date of Conference16/06/2017
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2017
LanguagesEnglish-United Kingdom

Last updated on 2018-10-05 at 16:45