3D Printing of Plant Golgi Stacks from Their Electron Tomographic Models
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AbstractThree-dimensional (3D) printing is an effective tool for preparing tangible 3D models from computer visualizations to assist in scientific research and education. With the recent popularization of 3D printing processes, it is now possible for individual laboratories to convert their scientific data into a physical form suitable for presentation or teaching purposes. Electron tomography is an electron microscopy method by which 3D structures of subcellular organelles or macromolecular complexes are determined at nanometer-level resolutions. Electron tomography analyses have revealed the convoluted membrane architectures of Golgi stacks, chloroplasts, and mitochondria. But the intricacy of their 3D organizations is difficult to grasp from tomographic models illustrated on computer screens. Despite the rapid development of 3D printing technologies, production of organelle models based on experimental data with 3D printing has rarely been documented. In this chapter, we present a simple guide to creating 3D prints of electron tomographic models of plant Golgi stacks using the two most accessible 3D printing technologies.
All Author(s) ListKeith Ka Ki Mai, Madison J. Kang, Byung-Ho Kang
All Editor(s) ListJiang Liwen
Book titlePlant Protein Secretion: Methods and Protocols
Series TitleMethods in Molecular Biology
Year2017
Month8
Volume Number1162
PublisherHumana Press, New York, NY
Pages105 - 113
ISBN978-1-4939-7261-6
eISBN978-1-4939-7262-3
LanguagesEnglish-United States
Keywords3D printing, Electron tomography, Golgi, Cell biology education

Last updated on 2020-20-10 at 01:08