Noncoding Centromeric RNA Expression Impairs Chromosome Stability in Human and Murine Stem Cells
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要We analyzed the effect of transcribed noncoding RNA centromeric satellites on chromosome segregation in normal human and murine stem and fibrosarcoma cells. The overexpression of different centromeric alphoid DNAs in all cell lines induced a marked increase in chromosome mis-segregation in anaphase. Overexpression of centromeric mouse minor satellite also increased chromosome instability in the murine stem but not in human cells. Analysis of chromosome segregation in vivo showed disturbances in the mitotic progression, which was frequently unresolved. Live cell imaging revealed that overexpression of centromeric satellites resulted in several different chromosomal morphological errors in the cell nuclei. Our findings correlated with other reports that several centromeric noncoding RNAs are detected in different carcinoma cells and their expression resulted in segregation errors. Our study furnishes further insights into a novel source of genomic instability in human and murine cells. It has recently been shown that noncoding centromeric RNAs are present in some form of cancer, and thus, overexpression of several types of centromeric noncoding RNAs may be useful as a specific maker for neoplastic cells.
出版社接受日期04.06.2017
著者David Y. L. Chan, Daniela Moralli, Suhail Khoja, Zoia L. Monaco
期刊名稱Disease Markers
出版年份2017
月份7
卷號2017
出版社Hindawi Ltd
出版地Netherlands
頁次7506976
國際標準期刊號0278-0240
電子國際標準期刊號1875-8630
語言美式英語

上次更新時間 2020-13-08 於 04:01