Effects of high progesterone level on the day of human chorionic gonadotrophin administration in in vitro fertilization cycles on epigenetic modification of endometrium in the peri-implantation period
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AbstractObjective

To investigate the effects of high P level on the day of hCG administration in IVF cycles on epigenetic modifications of endometrium in the peri-implantation period.

Design

Retrospective study.

Setting

University assisted reproductive unit.

Patient(s)

Forty patients were recruited: 20 women with high P levels were compared with 20 women with normal P levels.

Intervention(s)

Endometrial tissues were collected 7 days after hCG administration in women with normal or high P levels on the day of hCG administration. Immunohistochemical staining of DNA methylation (5-methylcytosine, 5-mC), histone methylation (H3K4me2/3, H3K9me2, H3K27me3), and histone acetylation (H3K4ac, H3K9ac) was performed.

Main Outcome Measure(s)

A semiquantitative analysis was performed in the luminal epithelium, glandular epithelium, and stroma, separately. Correlations between P, estrogen levels, and epigenetic markers were compared.

Result(s)

In luminal epithelium, the expression of H3K9me2 in the high P group was significantly higher than that in the normal P group. In glandular epithelium, the expression of 5-mC, H3K9me2, and H3K9ac in the high P group was significantly higher than that in the normal P group. In stroma, the expression of H3K27me3 in the high P group was significantly higher than that in the normal P group. Expression of 5-mC in glandular epithelium, H3k9me2 in glandular and luminal epithelium, and H3K27me3 in stroma was significantly correlated with P levels. H3k9me2 in glandular epithelium was significantly correlated with estrogen levels.

Conclusion(s)

High P level on the day of hCG administration in IVF cycles affects epigenetic modifications in three compartments of endometrium in the peri-implantation period. The epigenetic markers' expression was mainly correlated with P level. The altered epigenetic modification status in the endometrium may disrupt the endometrial receptivity in women with high P on the day of hCG administration.
All Author(s) ListXiong Yujing, Wang Jianzhang, Liu Liu, Chen Xiaoyan, Xu Hui, Li Tin Chiu, Wang Chi Chiu, Zhang Songying
Journal nameFertility and Sterility
Year2017
Month8
Volume Number108
Issue Number2
PublisherElsevier science inc
Place of PublicationUS
Pages269 - 276
ISSN0015-0282
eISSN1556-5653
LanguagesEnglish-United States
KeywordsEndometrium, epigenetics, progesterone

Last updated on 2020-15-10 at 02:45