Endoscopic Injection of Adipose-Derived Mesenchymal Stem Cells Enhanced Healing of Ulcers After Esd
Refereed conference paper presented and published in conference proceedings


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摘要Background & Aim
Our previous study showed that adipose-derived mesenchymal stem cells (ADMSCs) enhanced healing of perforated gastric ulcers in rat model. This study aimed to investigate the effect of endoscopic injection of ADMSCs on healing of NSAID related ulcers after endoscopic submucosal dissection (ESD) and its feasibility as an adjuvant to prevent ulcer complications.

Methods
Indomethacin (15mg/kg) was administered in all porcine models 10 days prior to ESD. We performed ESD at body and antrum of stomach in each pig under general anesthesia to create ulcer model. After ESD, one of the ulcers was treated by endoscopic injection of ADMSCs (1.0×107) at the submucosa, while the other ulcer was injected with PBS as control. Further endoscopic injection of ADMSCs and PBS was performed at respective ulcer one week after ESD. Indomethacin was continued after ESD till euthanasia as adjunctive agent to delay natural ulcer healing. Endoscopic surveillance was performed every 7 days to measure the ulcer size by an endoscopy ruler. The ulcer index was calculated as the product of the longest and shortest diameters of each lesion.

Results
Endoscopic surveillance demonstrated that ulcers receiving submucosal injection of ADMSCs had significantly faster healing than control; ulcer index was significantly reduced to 41% of the original size on day7 and 14% on day14 after ADMSCs injection therapy (Fig. A, B). Histological examination revealed good granulation and mucosal morphology in ulcer treated with ADMSCs on day 21 (Fig. C). Moreover, ADMSCs treatment significantly enhanced angiogenesis (Fig. D, E) and cellular proliferation (Fig. D, F) as well as increasing the expression of COX2 and VEGF in ulcer tissues (Fig. G, H).

Conclusion
Endoscopic submucosal injection of ADMSCs served as a novel approach to improve healing of large ulcers after ESD and indomethacin therapy.
著者Xia XF, Chiu PWY, Lam PK, Yeung BP, Wong GTY, Ng EK
會議名稱Digestive Disease Week 2017
會議開始日06.05.2017
會議完結日09.05.2017
會議地點Chicago, Illinois
會議國家/地區美國
會議論文集題名Gastrointestinal Endoscopy
出版作品名稱GASTROINTESTINAL ENDOSCOPY
出版年份2017
月份5
卷號85
期次5 Supplement
出版社Elsevier
頁次AB514 - AB514
國際標準期刊號0016-5107
語言英式英語
Web of Science 學科類別Gastroenterology & Hepatology;Gastroenterology & Hepatology

上次更新時間 2020-05-08 於 02:50