The Effects of Dithiothreitol on DNA
Publication in refereed journal

香港中文大學研究人員
替代計量分析
.

其它資訊
摘要With the novel possibilities for detecting molecules of interest with extreme sensitivity also comes the risk of encountering hitherto negligible sources of error. In life science, such sources of error might be the broad variety of additives such as dithiothreitol (DTT) used to preserve enzyme stability during in vitro reactions. Using two different assays that can sense strand interruptions in double stranded DNA, we here show that DTT is able to introduce nicks in the DNA backbone. DTT was furthermore shown to facilitate the immobilization of fluorescent DNA on an NHS-ester functionalized glass surface. Such reactions may in particular impact the readout from single molecule detection studies and other ultrasensitive assays. This was highlighted by the finding that DTT markedly decreased the signal to noise ratio in a DNA sensor based assay with single molecule resolution.
著者Søren Fjelstrup, Marie Bech Andersen, Jonas Thomsen, Jing Wang, Magnus Stougaard, Finn Skou Pedersen, Yi-Ping Ho, Marianne Smedegaard Hede, Birgitta Ruth Knudsen
期刊名稱Sensors
出版年份2017
月份5
日期24
卷號17
期次6
頁次1201
國際標準期刊號1424-8220
語言英式英語

上次更新時間 2020-28-11 於 01:49