Interlinked DNA nano-circles for measuring topoisomerase II activity at the level of single decatenation events
Publication in refereed journal

香港中文大學研究人員
替代計量分析
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其它資訊
摘要DNA nano-structures present appealing new means for monitoring different molecules. Here, we demonstrate the assembly and utilization of a surface-attached double-stranded DNA catenane composed of two intact interlinked DNA nano-circles for specific and sensitive measurements of the life essential topoisomerase II (Topo II) enzyme activity. Topo II activity was detected via the numeric release of DNA nano-circles, which were visualized at the single-molecule level in a fluorescence microscope upon isothermal amplification and fluorescence labeling. The transition of each enzymatic reaction to a micrometer sized labeled product enabled quantitative detection of Topo II activity at the single decatenation event level rendering activity measurements in extracts from as few as five cells possible. Topo II activity is a suggested predictive marker in cancer therapy and, consequently, the described highly sensitive monitoring of Topo II activity may add considerably to the toolbox of individualized medicine where decisions are based on very sparse samples.
著者Kristoffersen, Givskov, Jørgensen, Jensen, W Byl, Osheroff, Andersen, Stougaard, Ho, Knudsen
期刊名稱Nucleic acids research
出版年份2017
月份7
日期27
卷號45
期次13
頁次7855 - 7869
國際標準期刊號0305-1048
語言英式英語

上次更新時間 2020-24-11 於 01:25