Effects of high progesterone level on the day of human chorionic gonadotrophin administration in in-vitro fertilization cycles on epigenetic modification of endometriumin periimplantation period
Refereed conference paper presented and published in conference proceedings


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AbstractStudy question: Does high progesterone on the day of human chorionic gonadotrophin (hCG) administration in IVF cycles affect epigenetic modification of endometrium in the peri-implantation period?
Summary answer: DNA methylation, histone modification levels were increased in the endometrium of patients with high progesterone level on the day of hCG administration in IVF cycles.
What is known already: Epigenetic modification in the endometrium plays an important role in the regeneration, proliferation, angiogenesis and implantation, but the underlying mechanism is still not fully understood.
Study design, size, duration: A single-centre, retrospective cohort study between June 2013 and December 2013 included 40 infertile women, 20 women with normal progesterone and 20 women with high progesterone on
the day of hCG administration after controlled ovarian hyperstimulation in an in vitro fertilization cycles.
Participants/materials, setting, methods: Endometrial tissues were collected 7 days after hCG administration in women with normal or high progesterone levels on the day of hCG administration. Immunohistochemical staining
of DNA methylation (5-methylcytosine, 5-mC), histone methylation (H3K4me2/3, H3K9me2, H3K27me3) and histone acetylation (H3K4ac, H3K9ac) were performed.
Main results and the role of chance: In luminal epithelium, the expression of H3K9me2 in high progesterone group was significantly higher than that in normal progesterone group. In glandular epithelium, the expression of 5-mC,
fertilize, women in the P > 1.5 ng/mL group had a significantly lower number of embryos of sufficient quality to transfer or cryopreserve at both cleavage and blastocyst stage (49.6%, 48.0% and 43.1% of all fertilized oocytes were useable at blastocyst stage, respectively; p = 0.029). In the multivariable GEE regression models for live birth, fresh LBR varied significantly (29.3%, 34.3% and 25.5%, respectively; p = 0.0029) among the groups. Most importantly, while both women with P < 0.5 and >1.5 ng/mL had similarly low cumulative LBR (38.8%, 43.9% and 38.3%, respectively; p = 0.0211), only the difference between the women with P levels between 0.5-1.5 ng/mL and >1.5 ng/mL remained significant after pairwise comparison.
Limitations, reasons for caution: The main limitation of the study was its retrospective nature. Furthermore, the analysis was restricted to patients under GnRH antagonist pituitary suppression and some potential confounding factors such as female smoking could not be accounted for.
Wider implications of the findings: Our results are in contrast with previous studies suggesting that high P is not associated with reduced embryo quality or cumulative LBR. The knowledge that high P levels may hinder oocyte/ embryo competence and, ultimately, diminish cumulative LBR could play an important role in how we perform ovarian stimulation henceforth.
All Author(s) ListXiong YJ, Wang JZ, Liu L, Chen XY, Li TC, Wang CC, Zhang SY
Name of ConferenceThe 33rd Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE)
Start Date of Conference02/07/2017
End Date of Conference05/07/2017
Place of ConferenceGeneva
Country/Region of ConferenceSwitzerland
Proceedings TitleHuman Reproduction
Year2017
Month7
Volume Number32
Issue NumberS1
LanguagesEnglish-United States

Last updated on 2018-18-01 at 11:16