Is the peripheral blood transcriptome profile 9 days after blastocyst transfer in pregnancy destined to miscarriage different from pregnancy which subsequently progressed to live birth?
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AbstractStudy question: Are there any differences in the peripheral blood transcriptome profiles 9 days after single blastocyst transfer between pregnancies whichsubsequently progressed to live birth or miscarriage?
Summary answer: There were significant differences in peripheral blood transcriptomic profile 9 days after single blastocyst transfer between pregnancies which subsequently progressed to live birth or miscarriage.
What is known already: In women who received IVF treatment, serum human chorionic gonadotrophin (hCG) measurement 14 days after oocyte retrieval or 9 days after blastocyst transfer can establish if pregnancy has
occurred. The majority of pregnancies progress to live birth but some end up in miscarriage. Women who later miscarried appeared to have lower serum hCG level 9 days after blastocyst transfer compared with those which progressed to live birth. However, there is significant overlap in hCG levels between the two groups for the hCG measurement to be of significant discriminatory value.
Study design, size, duration: This is a prospective, cross-sectional, observational
study. 100 women who underwent IVF treatment had blood sample taken 9 days after blastocyst transfer for hCG measurement and a portion of
the blood sample stored for later transcriptome study. Six samples from women whose pregnancy progressed to live birth and 6 women whose pregnancy later miscarried were randomly chosen for transcriptome study.
Participants/materials, setting, methods: Total RNA were extracted from peripheral blood, and purified for RNA sequencing. Differential gene expression and transcriptome patterns were studied by using DESeq2 package.
Main results and the role of chance: The two groups had comparable hCG levels 9 days after blastocyst transfer but there were 143 significantly differential expressed genes in the miscarriage group. These deferentially
expressed genes were mainly enriched in the immune related pathways.
Limitations, reasons for caution: Quantitative PCR study is in progress to validate the differentially expressed genes.
Wider implications of the findings: Peripheral blood transcriptome study 9 days after blastocyst may reflect the success or failure of the implantation process. The panel of differentially expressed genes may be used to predict the
outcome of pregnancy.
All Author(s) ListHuang J, Jin N, Zhu W, Cheung W, Liu Y, Wang CC, Chan TF, Li TC
Name of ConferenceThe 33rd Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE)
Start Date of Conference02/07/2017
End Date of Conference05/07/2017
Place of ConferenceGeneva
Country/Region of ConferenceSwitzerland
Proceedings TitleHuman Reproduction
Year2017
Month7
Volume Number32
Issue NumberS1
PublisherOxford Open
LanguagesEnglish-United States

Last updated on 2020-18-10 at 00:50