Uterine natural killer (uNK) cell number in the periimplantation endometrium of normal fertile women and women with recurrent reproductive failure
Refereed conference paper presented and published in conference proceedings


摘要Summary answer: No consistent effect was found in the gene expression in the paired endometrial samples prior to and after polypectomy.
What is known already: Endometrial polyps may negatively impact endometrial receptivity through various mechanisms, and surgical removal of the polyps may improve fertility outcome in subfertile patients. Some studies
showed that hysteroscopic polypectomy reduces nuclear factor-kappa B expression, increases concentrations of insulin-like growth factor binding protein-1, tumour necrosis factor α and osteopontin in the endometrium. No
published study has examined the effect of hysteroscopic polypectomy on global gene expression, and we aimed at studying this.
Study design, size, duration: This was a prospective observational study in a university-affiliated hospital. We recruited 9 subfertile women who were found to have endometrial polyp on saline infusion sonohysterogram and
scheduled for hysteroscopic polypectomy before in-vitro fertilisation.
Participants/materials, setting, methods: For each patient, endometrial biopsies were taken before and after hysteroscopic polypectomy 7 days after luteinizing hormone surge (LH+7). We compared the gene expression profiles of the paired endometria from 3 women using Affymetrix Gene 2.0 ST Array. Quantitative-PCR on TaqMan assays was used to confirm the microarray result. Furthermore, 8 selected receptivity genes were used to compare the gene expression profiles with natural (LH+7, n = 8) and stimulated (hCG+7, n = 8, excessive responders) endometrial samples.
Main results and the role of chance: Microarray analysis demonstrated that no genes were commonly differentially expressed in all 3 paired samples. The expression of 16 genes (hERα, hERβ, OLFM1, OLFM2, IL15, CXCL14,
GPX3, CCL4, UPKIB, PAEP, MMP26, SLC1A1, MUC15, MUC16, TM4SF4 and TFPI2) that were differentially expressed (≥2-fold) in 2 of the 3 paired samples were confirmed with quantitative-PCR in all 9 paired samples. No consensus change on 8 receptive genes (OLFM1, IL15, CXCL14, GPX3, PAEP, MMP26, SLC1A1 and MUC16) expression was found after polypectomy when compared to endometrial samples taken on LH+7 or hCG+7. Limitations, reasons for caution: The main limitation of the present study was the small sample size.
Wider implications of the findings: This was the first study in the literature evaluating the effect of hysteroscopic polypectomy on endometrial receptivity by microarray evaluation of global gene expression. Polypectomy may modulate other endometrial environment conducive to embryo implantation. Further study with larger sample size is needed.
著者Chen XY, Jiang L, Liu Y, Mariee N, Wang CC, Laird S, Li TC
會議名稱The 33rd Annual Meeting of the European Society of Human Reproduction and Embryology (ESHRE)
會議論文集題名Human Reproduction
期次Suppl. 1
頁次i107 - i108

上次更新時間 2020-23-11 於 00:57