The anti-inflammatory effects of pentaherbs formula on ovalbumin-induced asthmatic mice model
Invited conference paper presented and published in conference proceedings


其它資訊
摘要Introduction
Allergic asthma is an inflammatory airway disease, affecting as many as 334 million people worldwide. Inhaled steroids have long been used as the major treatment of asthma. Yet, a long-term use of steroids may bring undesirable effects. Previous studies show that pentaherbs formula (PHF), which consists of five traditional Chinese herbal medicines, flos lonicerae, herba menthae, cortex phellodendri, cortex moutan and rhizoma atractylodis at w/w ratio of 2:1:2:2:2 exhibited therapeutic potential in treating oxazolone-induce datopic dermatitis-like mice model.

Objectives
As allergic asthma and atopic dermatitis are immunologically related to each other, we hypothesize that PHF also poses potential anti-inflammatory and immunomodulatory activities on asthmatic mice. This study aims at investigating the potential anti-inflammatory and immunomodulatory activities of PHF in asthmatic mice model.

Methods
Acute asthmatic mouse models were established by sensitizing the BALB/c mice with an immunogen 20 µg ovalbumin (OVA) - Al(OH)3 by i.p. administration on day 1 and day 15; followed by challenging the mice with the same immunogen on day 27, 28 and 29. PHF water extract (920 mg/kg/day) was administered to the mice by intragastrical delivery twice a day on day 1, 2, 3, 15, 16, 17, and/or from day 22 to 29. One day after the last challenge, the airway hyper-responsiveness was measured in the enhanced pause (penh) value with Buxco Whole Body Plethysmography. Serum were collected for the determination of total IgE and OVA-specific IgE, and inflammatory cytokines and chemokine by ELISA and Multiplex assay,respectively.

Results
Upon a 50 mg/mL methacholine challenge, the penh value of the asthmatic mice under a 14-day PHF treatment is significantly improved when compared to the sham group (p<0.05). However, an eight-day PHF treatment did not affect the airway hyperresponsiveness of the mice (p<0.05). Besides, intragastrical administration of PHF to the mice model twice a day does not affect the weight of the mice.

Conclusions
In this study, pentaherbs formula was found to improve the airway hyperresponsiveness of ovalbumin-induced asthmatic mice, indicating the potential use of PHF as a supplement for treating allergic diseases.
出版社接受日期13.03.2017
著者Tsang S.M., Zhu J., Liu D., Shaw P.C., Hon K.L., Leung P.C., Wong C.K.
會議名稱EAACI Congress 2017
會議開始日17.06.2017
會議完結日21.06.2017
會議地點Helsinki
會議國家/地區芬蘭
出版年份2017
月份6
語言英式英語

上次更新時間 2018-18-01 於 11:18