Chromosome Preparation for Myeloid Malignancies
Publication in refereed journal

香港中文大學研究人員

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摘要Many cases of myeloid malignancies are associated with recurring cytogenetic aberrations. Chromosomal analysis can aid in diagnosis, predict prognosis, and disclose subsequent clonal evolution. Three different cell culture methods: direct harvest, nonsynchronized culture, and synchronized culture are usually prepared if the nucleated cell counts in marrow blood are sufficient. Synchronized culture is the first choice of method in myeloid malignancies, whereas the direct method can be omitted if the cell count is low. The aseptic culture technique is strictly followed until harvesting procedure. For synchronized culture, uridine and fluorodeoxyuridine are added as blocking reagents and released by thymidine on the following day. Harvesting steps of the cultures involved colcemid exposure, hypotonic treatment, and Carnoy's fixation. The cells are then ready for slide making and banding for chromosomal analysis.
著者Hui EK, Wan TS, Ng MH
期刊名稱Methods in molecular biology (Clifton, N.J.)
詳細描述Part of the Methods in Molecular Biology book series
出版年份2017
卷號1541
頁次11 - 17
國際標準書號978-1-4939-6701-8
國際標準期刊號1064-3745
語言英式英語

上次更新時間 2021-01-03 於 01:55