Herbal medicine for liver protection in experimental animals-a histochemical, pathological study
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AbstractPien Tze Huang (PTH) is a mixture of several agents of traditional medicine prescribed for over 600 years ago by
a retired Court Physician, for conditions of liver diseases in southern China. For all these hundreds of years to the
present day, PTH traditional medicine has been widely taken orally by the ethnic communities not only in China
but also in the Southeast Asia as a hepatoprotective drug. Previous animal studies on this traditional drug
revealed that it could indeed protect liver damage against carbon tetrachloride (CCl4) insults in mice model and
hepatoma culture. After drug treatment of PTH, increased level of markers responsible to tackle superoxide
formation and upregulated immune activities were evident [1]. The present study attempted to evaluate the
further hepatoprotective effect of this drug against bile duct ligation and acute intoxication of alcohol in the mice
model, employing biochemistry and histochemistry, along with histopathological observations. After bile duct
ligation and acute alcohol treatment, both lactic dehydrogenase (LDH) positive cells (indicating necrosis) and
terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive cells (indicating apoptosis)
went up in these experimental animals, along with an increase in transaminase. Histopathological observation
indicated increase in fibrosis starting 10 days after bile duct ligation or 7 days after acute alcohol model.
However, in those animals with PTH treatment which had either bile duct ligation or acute alcohol treatment, the
number of necrotic and apoptotic cells decreased when compared with those experimental animals without PTH
treatment. Concurrently, there was also a downregulation of the transaminase and the extent of fibrosis after PTH
treatment in the experimental animals of both ligation and alcohol treated groups. Transforming growth factor
beta (TGF-beta) positive cells also increased in number in the bile duct ligated mice but not in the acute alcohol
treated mice. In the former group, PTH treatment was effective in the downregulation of the increase of TGFbeta
cells in the liver. In addition, PTH treatment enhanced the proliferation of cells in the liver after bile duct
ligation and acute alcohol treatment, thus helpful in regeneration as well. On the whole, the PTH treatment which
had been used for more than 600 years appeared to have definitive protective effects on the liver after injury as
demonstrated in this study of the mice model.
All Author(s) ListF. Hong, Z. Chen, H.C. Tang, Sharon L. Wu, Jingjing Yang, David T. Yew
All Editor(s) ListF. Hong, Z. Chen, H.C. Tang2, S. L. Wu, M.S.M. Wai, D.T. Yew
Book titleMicroscopy and imaging science: practical approaches to applied research and education
PublisherFormatex Research Center
Place of PublicationSpain
LanguagesEnglish-United Kingdom
Keywordstraditional Chinese medicine, Pien Tze Huang, liver, hepatoprotective, histochemistry

Last updated on 2018-20-01 at 18:17