The flavone apigenin blocks SREBP-2 activation in hepatic cells
Refereed conference paper presented and published in conference proceedings

香港中文大學研究人員
替代計量分析
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其它資訊
摘要SREBP-2 is a pivotal transcriptional factor in cholesterol metabolism. Factors interfering with the proper functioning of SREBP-2 potentially alter plasma lipid concentrations. Consuming fruits and vegetables is associated with beneficial plasma lipid profile. The mechanism by which plant foods induce desirable lipid changes remains unclear. Apigenin, a common plant food flavonoid, was shown to prevent the nuclear translocation of SREBP-2 in the hepatic cells WRL and HepG2 in the current study. The processing of SREBP-2 protein occurred after translation, and apigenin blocked this activation route. Further study indicated that AMPK was activated by the flavone and co-administrating the AMPK-specific inhibitor compound C could release the blockage. Reporter gene assay revealed that the transactivation of SRE-containing HMGCR promoter was suppressed by the flavone. Similarly, EMSA result also demonstrated a reduced DNA-binding activity on the SRE domain under the same treatment. The reduced transactivity and DNA-binding activity could be attributed to a decreased amount of SREBP-2 translocating from cytosol to nucleus as depicted by confocal microscopy. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay demonstrated that the transcription of HMGCR followed the same pattern of SREBP-2 translocation. In summary, the present study showed that apigenin modulated SREBP-2 translocation and reduced the downstream gene HMGCR transcription.
著者WONG Tsz Yan, LIN Shu-mei, LEUNG Lai Kwok
會議名稱GA 2015 - 63rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research
會議開始日23.08.2015
會議完結日27.08.2015
會議地點Budapest
會議國家/地區德國
會議論文集題名PLANTA MEDICA
詳細描述the Society for Medicinal Plant and Natural Product Research,\n\nTo ORKTS: Planta Med 2015; 81 - PM_73
DOI: 10.1055/s-0035-1565450
出版年份2015
月份11
卷號81
期次16
出版地Germany
頁次1444 - 1444
國際標準期刊號0032-0943
電子國際標準期刊號1439-0221
語言英式英語
關鍵詞apigenin; SREBP-2; liver

上次更新時間 2020-21-09 於 02:18