The flavone apigenin blocks SREBP-2 activation in hepatic cells
Refereed conference paper presented and published in conference proceedings

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AbstractSREBP-2 is a pivotal transcriptional factor in cholesterol metabolism. Factors interfering with the proper functioning of SREBP-2 potentially alter plasma lipid concentrations. Consuming fruits and vegetables is associated with beneficial plasma lipid profile. The mechanism by which plant foods induce desirable lipid changes remains unclear. Apigenin, a common plant food flavonoid, was shown to prevent the nuclear translocation of SREBP-2 in the hepatic cells WRL and HepG2 in the current study. The processing of SREBP-2 protein occurred after translation, and apigenin blocked this activation route. Further study indicated that AMPK was activated by the flavone and co-administrating the AMPK-specific inhibitor compound C could release the blockage. Reporter gene assay revealed that the transactivation of SRE-containing HMGCR promoter was suppressed by the flavone. Similarly, EMSA result also demonstrated a reduced DNA-binding activity on the SRE domain under the same treatment. The reduced transactivity and DNA-binding activity could be attributed to a decreased amount of SREBP-2 translocating from cytosol to nucleus as depicted by confocal microscopy. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) assay demonstrated that the transcription of HMGCR followed the same pattern of SREBP-2 translocation. In summary, the present study showed that apigenin modulated SREBP-2 translocation and reduced the downstream gene HMGCR transcription.
All Author(s) ListWONG Tsz Yan, LIN Shu-mei, LEUNG Lai Kwok
Name of ConferenceGA 2015 - 63rd International Congress and Annual Meeting of the Society for Medicinal Plant and Natural Product Research
Start Date of Conference23/08/2015
End Date of Conference27/08/2015
Place of ConferenceBudapest
Country/Region of ConferenceGermany
Proceedings TitlePLANTA MEDICA
Detailed descriptionthe Society for Medicinal Plant and Natural Product Research,\n\nTo ORKTS: Planta Med 2015; 81 - PM_73
DOI: 10.1055/s-0035-1565450
Volume Number81
Issue Number16
Place of PublicationGermany
Pages1444 - 1444
LanguagesEnglish-United Kingdom
Keywordsapigenin; SREBP-2; liver

Last updated on 2021-23-09 at 00:48