Protective Effects of Verbascoside on Statin-induced Myotoxicity in Zebrafish Embryos
Refereed conference paper presented and published in conference proceedings


其它資訊
摘要Myotoxicity is a well-known side effect of statins, the common lipid-lowering drug. Herba Cistanches (HC, Cistanche deserticola) is a Chinese herb traditionally used for muscle problems. Recent studies demonstrated that HC could reduce muscle damage in post-exercised rats. Verbascoside (Vb) was found as the major constituent in the active fraction of HC and is known to be muscle protective, though its effect on statin-induced muscle toxicity has never been investigated. The aim of this study was to investigate the protective effect of Vb on simvastatin-induced
myotoxicity using zebrafish embryos model. Embryos treated with simvastatin (0 -0.5 µM) at 24 hpf, with or without co- treatment of Vb (0.32 mM, maximum non-cytotoxic dose) were fixed at 48 hpf, and whole muscles were stained using the anti–slow twitch myosin F59 antibody. For each treatment group, the diameters of at least 300 muscle fibers were measured and represented as ratio of mean fiber size against control. Results showed that muscle damage at simvastatin concentrations (0.4 and 0.5 µM) were evidenced by bowing, thin and irregular fibers
with diffuse appearance, while these damages were significantly improved by the co-treatment of Vb. Simvastatin caused significant dose-dependent reduction on myotube diameter as compared to control (61.5 -73.8% vs 100%). Vb (0.32 mM) significantly (p<0.001) prevented the simvastatin-induced reduction in myotube diameter at 0.4
µM (104.7 vs 73.8%) and 0.5 µM (84.6 vs 61.5%) of simvastatin concentrations. In conclusion, our study demonstrated for the first time that verbascoside could protect against simvastatin-induced myotoxicity in vivo.
Acknowledgement: This work was financially supported by Food and Health Bureau HKSAR, Health and Health
Services Research Fund no. 11120831.
著者WAT Chi Ling Elaine, KWOK Hin Fai, NG Chun Fai, KOON Chi Man, SALLE DE CHOU Y, FERRIERE Jessica, TOMLINSON Brian, LAU Bik San Clara
會議名稱Joint Meeting of the GP-TCM Research Association, The TCM Chemistry Specialty Committee and The TCM Pharmaceutical Analysis Specialty Committee of the World Federation of Chinese Medicine Societies
會議開始日13.07.2015
會議完結日15.07.2015
會議地點Mons
會議國家/地區比利時
會議論文集題名World Journal of Traditional Chinese Medicine
出版年份2015
月份7
日期13
卷號1
期次4
出版地Belgium, Mons
語言英式英語

上次更新時間 2018-27-06 於 10:48