Novel Anti-angiogenic Activities of a Triterpenoid Deoxyactein from Cimicifuga foetida
Refereed conference paper presented and published in conference proceedings

摘要Deoxyactein (DA) is a triterpenoid isolated from the root of Cimicifuga foetida which is a source of traditional Chinese herb “shengma” and is traditionally used as an antipyretic and analgesic agent. Previous studies showed that DA exhibited anti-proliferative effect in human breast cancer cells. Hence, the objectives of this study were to investigate the in vitro and in vivo effects of DA on angiogenesis using human microvascular endothelial cells (HMEC-1) and matrigel plug mouse model, respectively. The proliferation, motility and invasion of endothelial cells were assessed by thymidine incorporation, scratch wound and transwell migration assays, respectively. The mRNA expressions of angiogenic receptors in DA-treated endothelial cells were also examined. Our results showed that DA (3.125 -100 mM) significantly inhibited the proliferation of HMEC-1 without cytotoxicity. The motility and invasion of endothelial cells were also inhibited by 10 – 40 mM DA. Besides, this compound could down-regulate the mRNA expressions of VEGFR1 and VEGFR3 in HMEC-1 cells. Results on matrigel plug mouse model showed that oral administration of DA at 20 mg/kg for 5 days inhibited the blood vessel growth in the growth factor-containing matrigel plugs. The hemogloblin content in the plugs was significantly reduced in DA-treated mice. This is the first report of the antiangiogenic activities of DA in human endothelial cells and in mouse model. Our findings indicate that DA may act as another bioactive component in Cimicifuga species. Acknowledgement: This work was financially supported by the National Natural Science Foundation of China (U1132604 and 81302670).
著者YUE Gar Lee Grace, LEE Kin Ming, KWOK Hin Fai, NIAN Yin, QIU Ming-hua, LAU Bik San Clara
會議名稱Joint Meeting of the GP-TCM Research Association, The TCM Chemistry Specialty Committee and The TCM Pharmaceutical Analysis Specialty Committee of the World Federation of Chinese Medicine Societies
會議論文集題名World Journal of Traditional Chinese Medicine
出版地Belgium, Mons

上次更新時間 2018-27-06 於 10:40