A Biflavonoid Amentoflavone from Garcinia xanthochymus Fruit Exhibited Anti-angiogenic Activities in Zebrafish Embryos
Refereed conference paper presented and published in conference proceedings

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AbstractEdible fruits of Garcinia xanthochymus were traditionally used to treat diarrhoea and dysentery. Our previous work showed that some components of G. xanthochymus fruits induced apoptosis in colon cancer cells. In this study, the aril (edible pulp of the fruit) and seed of G. xanthochymus fruits were further investigated for their potential antiangiogenesis effects, with an aim to evaluate the anti-angiogenic effect of the crude methanolic extract and isolated compounds using transgenic zebrafish Tg(fli1a:EGFP)y1 with fluorescent blood vessels. The growth of sub intestinal vessels (SIV) and gene expression in angiogenesis signaling pathways of extract/compound-treated zebrafish embryos were examined. Our initial results showed that the methanolic extract from the aril (15 mg/mL), but not the seed, showed promising inhibitory effect on the growth of SIV in zebrafish embryos. LC-MS analysis suggested that certain Garcinia polyphenols correlated with the activity. Three representative compounds (alloathyriol, xanthochymol and amentoflavone) from 3 classes of constituents (xanthone, catechol and flavonoid) were further examined for their activities in zebrafish embryos. Results showed that amentoflavone (10-20 mM), but not alloathyriol and xanthochymol, could significantly inhibit the growth of SIV. It also down-regulated the expressions of Angtp2 and Tie2 genes of zebrafish embryos. A detectable amount of amentoflavone in aril was demonstrated using LC-MS-TQD which may account for the anti-angiogenic effects. In conclusion, this is the first report of the anti-angiogenic activities of amentoflavone in zebrafish embryos. These findings suggested the anti-angiogenic potential of this plant species and may lead to further exploration of active constituents in different plant parts.
All Author(s) ListYUE Gar Lee Grace, KWOK Hin Fai, LI Ping, SENTHILKUMAR Harini A, LONG Chun-lin, KENNELLY Edward J., LAU Bik San Clara
Name of ConferenceJoint Meeting of the GP-TCM Research Association, The TCM Chemistry Specialty Committee and The TCM Pharmaceutical Analysis Specialty Committee of the World Federation of Chinese Medicine Societies
Start Date of Conference13/07/2015
End Date of Conference15/07/2015
Place of ConferenceMons
Country/Region of ConferenceBelgium
Proceedings TitleWorld Journal of Traditional Chinese Medicine
Volume Number1
Issue Number4
Place of PublicationBelgium, Mons
LanguagesEnglish-United Kingdom

Last updated on 2021-07-05 at 11:12