Identification of herbal components in decoctions and medicinal granules by DNA markers
Refereed conference paper presented and published in conference proceedings

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AbstractGenomic DNA provides a rich source of material for species identification. However, molecular authentication is usually regarded as unsuitable for herbal decoctions and medicinal granules, as DNA is highly degraded. We set forth to investigate the availability of DNA in these products using PCR and DNA sequencing. Decoctions with Panax ginseng (Ginseng) or P. quinquefolius (American ginseng), two classical ginseng prescriptions with multiple herbal materials, and a commercial ginseng soup were tested. The DNA extraction procedure, sample pulverization, and boiling time were examined. Results showed: (1) Sample pulverization before boiling improved PCR detection. (2) Prolonged boiling increased the DNA concentration but decreased the intactness of DNA. (3) Ginseng could be differentiated from American ginseng by multiplex PCR. (4) Individual herbs in the decoctions with prolonged boiling time were identified by amplifying short DNA fragments. We also demonstrated the presence of DNA in a number of animal and plant medicinal granules and they could be amplified by species-specific primers.
All Author(s) ListSHAW Pang Chui, LO Yat Tung, JIANG Lili
Name of Conference15th Annual Oxford International Conference on the Science of Botanicals
Start Date of Conference13/04/2015
End Date of Conference16/04/2015
Place of ConferenceMississippi
Country/Region of ConferenceUnited States of America
Proceedings TitleAbstracts of the 15th International Conference on the Science of Botanicals
Detailed descriptionorganized by National Center for Natural Products Research
Volume Number81
Issue Number05
PublisherPlanta Medica
PagesOA38 - OA38
LanguagesEnglish-United Kingdom

Last updated on 2021-23-09 at 00:48