Detection of MicroRNA-135b in Stool of Patients with Advanced Neoplasm: A Potential Non-invasive Screening Biomarker?
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AbstractBackground: Stool molecular markers, MicroRNAs (miRNA) represent a non-invasive approach to screen for colorectal cancer, but their role in the detection of precancerous lesions in the colon is unclear. We investigated the miRNA expression profile of advanced neoplasms (AN) and further test these markers in stool samples of subjects with high risk adenomas. Methods: Biopsies were obtained during colonoscopy from 18 AN (adenomas >10mm size, >25% villous features, severe dysplasia or carcinoma-in-situ) and their adjacent normal tissues. Expression of miRNA was screened in 2 pairs of AN and adjacent normal tissues using TaqMan Human microRNA array assay (covering 667 human miRNAs). Stool samples were collected from patients with AN (n=22), any other adenomas (n=54), inflammatory bowel disease (n=42) and normal controls (n=42). MiRNA levels in tissue or stool samples were detected by quantitative real-time PCR. Results: Five miRNAs were upregulated (miR-31, miR-200b, miR-649, miR-1254, miR-135b; > 4-fold increased; p<0.001) whilst four miRNA were down-regulated (miR-215, miR-495, miR-582, miR-1243; >5-fold decrease; p<0.001) in AN compared with adjacent normal tissues. Expression of miR-135b (22.5-fold) and miR-31 (31.2-fold) but not miR-200b (1.57-fold), miR-649 (1.15-fold), and miR-1254 (1.12-fold) was significantly higher in 18 pairs of adenoma tissues compared with adjacent normal tissues (p<0.0001). Patients with AN [median, 47.92; IQR: 14.95-150.4)] had significantly higher stool miR-135b levels compared with patients with adenomas (median, 25.89; IQR: 0-57.15) and controls (median, 25.57; IQR: 0-48.61; p<0.05; Figure 1). Stool miR-135b had a sensitivity of 63.64% and a specificity of 66.67% for the diagnosis of AN (Figure 2). Conclusion: We have demonstrated the feasibility of detecting aberrant miRNA expression in stool samples of patients with AN. Stool miR-135b is a potential novel non-invasive biomarker for AN. (Table Presented).AN - WOS:000306994300129
All Author(s) ListNG Siew Chien, LAW Wai Tak, WU Chung-wah, SUEN BY, LAU Yun Wong, CHAN Francis K.L., SUNG Joseph Jao-yiu, YU Jun
Name of ConferenceThe Digestive Disease Week 2012
Start Date of Conference19/05/2012
End Date of Conference22/05/2012
Place of ConferenceSan Diego
Country/Region of ConferenceUnited States of America
Proceedings TitleGastroenterology
Volume Number142
Issue NumberSupplement 1
PagesS32 - S33
LanguagesEnglish-United States

Last updated on 2021-10-10 at 00:14