Delayed ACL reconstruction has no significant impact on ACL graft healing compared with timezero reconstruction in a non-invasive ACL rupture rat model
Refereed conference paper presented and published in conference proceedings
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摘要Introduction: Anterior cruciate ligament reconstruction (ACLR) is performed between 4-10 weeks post-injury clinically, coinciding with the subsided acute inflammatory response. However, present animal models, used for studying ACL graft healing, engage in immediate reconstruction post-ACL dissection, potentially introducing a significant bias within preclinical assessments.
Methods: Male 12-week-old Sprague Dawley (S-D) rats were randomly assigned to two groups: (1) immediate
reconstruction group (immediate group) and (2) delayed reconstruction group (Delay group) [n=3/group]. Non-invasive methods were employed for ACL rupture, and ACLR was performed either immediately or one-week post-rupture. Histological assessments of ACLR graft healing were conducted two weeks post-reconstruction surgery.
Results: 85.3±6.5 N single over-load force could induce ACL rupture in SD rats. Inflammation and healing of the distal femoral tunnel were slightly better in the Delay group compared with the immediate group at 2 weeks. However, deterioration of intra-articular grafts was not observed.
Discussion and Conclusion: Preliminary findings suggest that delayed reconstruction, post-ACL rupture, may not
significantly enhance graft healing within the rat model. The credibility of the established ACLR rat model remains unaffected due to varying time frames of reconstruction. Further investigations integrating larger sample sizes, biomechanical analyses, and in-situ inflammatory factor detection are indispensable for solidifying the conclusion.
Methods: Male 12-week-old Sprague Dawley (S-D) rats were randomly assigned to two groups: (1) immediate
reconstruction group (immediate group) and (2) delayed reconstruction group (Delay group) [n=3/group]. Non-invasive methods were employed for ACL rupture, and ACLR was performed either immediately or one-week post-rupture. Histological assessments of ACLR graft healing were conducted two weeks post-reconstruction surgery.
Results: 85.3±6.5 N single over-load force could induce ACL rupture in SD rats. Inflammation and healing of the distal femoral tunnel were slightly better in the Delay group compared with the immediate group at 2 weeks. However, deterioration of intra-articular grafts was not observed.
Discussion and Conclusion: Preliminary findings suggest that delayed reconstruction, post-ACL rupture, may not
significantly enhance graft healing within the rat model. The credibility of the established ACLR rat model remains unaffected due to varying time frames of reconstruction. Further investigations integrating larger sample sizes, biomechanical analyses, and in-situ inflammatory factor detection are indispensable for solidifying the conclusion.
著者Mingde Cao, Xueyou Zhang, Patrick Shu Hang Yung, Michael Tim Yun Ong
會議名稱The HKOA 43rd Annual Congress of The Hong Kong Orthopaedic Association
會議開始日04.11.2023
會議完結日05.11.2023
會議地點Hong Kong
會議國家/地區香港
出版年份2023
月份11
出版社Hong Kong Academy of Medicine Press
出版地Hong Kong
頁次138 - 138
語言美式英語
