Delayed ACL reconstruction has no significant impact on ACL graft healing compared with timezero reconstruction in a non-invasive ACL rupture rat model
Refereed conference paper presented and published in conference proceedings
CUHK Authors
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AbstractIntroduction: Anterior cruciate ligament reconstruction (ACLR) is performed between 4-10 weeks post-injury clinically, coinciding with the subsided acute inflammatory response. However, present animal models, used for studying ACL graft healing, engage in immediate reconstruction post-ACL dissection, potentially introducing a significant bias within preclinical assessments.
Methods: Male 12-week-old Sprague Dawley (S-D) rats were randomly assigned to two groups: (1) immediate
reconstruction group (immediate group) and (2) delayed reconstruction group (Delay group) [n=3/group]. Non-invasive methods were employed for ACL rupture, and ACLR was performed either immediately or one-week post-rupture. Histological assessments of ACLR graft healing were conducted two weeks post-reconstruction surgery.
Results: 85.3±6.5 N single over-load force could induce ACL rupture in SD rats. Inflammation and healing of the distal femoral tunnel were slightly better in the Delay group compared with the immediate group at 2 weeks. However, deterioration of intra-articular grafts was not observed.
Discussion and Conclusion: Preliminary findings suggest that delayed reconstruction, post-ACL rupture, may not
significantly enhance graft healing within the rat model. The credibility of the established ACLR rat model remains unaffected due to varying time frames of reconstruction. Further investigations integrating larger sample sizes, biomechanical analyses, and in-situ inflammatory factor detection are indispensable for solidifying the conclusion.
Methods: Male 12-week-old Sprague Dawley (S-D) rats were randomly assigned to two groups: (1) immediate
reconstruction group (immediate group) and (2) delayed reconstruction group (Delay group) [n=3/group]. Non-invasive methods were employed for ACL rupture, and ACLR was performed either immediately or one-week post-rupture. Histological assessments of ACLR graft healing were conducted two weeks post-reconstruction surgery.
Results: 85.3±6.5 N single over-load force could induce ACL rupture in SD rats. Inflammation and healing of the distal femoral tunnel were slightly better in the Delay group compared with the immediate group at 2 weeks. However, deterioration of intra-articular grafts was not observed.
Discussion and Conclusion: Preliminary findings suggest that delayed reconstruction, post-ACL rupture, may not
significantly enhance graft healing within the rat model. The credibility of the established ACLR rat model remains unaffected due to varying time frames of reconstruction. Further investigations integrating larger sample sizes, biomechanical analyses, and in-situ inflammatory factor detection are indispensable for solidifying the conclusion.
All Author(s) ListMingde Cao, Xueyou Zhang, Patrick Shu Hang Yung, Michael Tim Yun Ong
Name of ConferenceThe HKOA 43rd Annual Congress of The Hong Kong Orthopaedic Association
Start Date of Conference04/11/2023
End Date of Conference05/11/2023
Place of ConferenceHong Kong
Country/Region of ConferenceHong Kong
Year2023
Month11
PublisherHong Kong Academy of Medicine Press
Place of PublicationHong Kong
Pages138 - 138
LanguagesEnglish-United States