Quantitative comparison of the renal pelvic urine and bladder urine to examine modifications of the urine proteome by the lower urinary tract
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摘要Purpose: Urine proteome is a valuable reservoir of biomarkers for disease diagnosis and monitoring. Following formation as the plasma filtrate in the kidney, urine is progressively modified by the active reabsorption and secretion of the urinary tract. However, little is known about how the urine proteome changes as it passes along the urinary tract. Experimental design: To investigate this, we compared the proteome composition of the renal pelvis urine (RPU) and individually self-voided bladder urine (BU) collected from seven unilateral urinary tract obstruction male patients by LC-MS/MS screening. To our knowledge, this is the first proteomic comparison of RPU and BU samples from the same individual. Results: Overall, RPU and BU proteomes did not exhibit proteins that were exclusively present in all samples of one urine type while in none of the other type. Nonetheless, BU had more overrepresented proteins that were observed at a higher frequency than RPU. Label-free quantitative analyses revealed BU–RPU differential proteins that are enriched in exosomes and extracellular proteins. However, the differences were not significant after corrections for multiple testing. Interestingly, we observed a significant increase of collagen peptides with hydroxyproline modifications in the BU samples, suggesting differences in protein modifications. Conclusions and clinical relevance: Our study revealed no substantial differences at the protein level between the BU and RPU samples. Future investigations with expanded cohorts would provide more insights about the urothelial–urinary interactions.
出版社接受日期04.08.2023
著者Pan Y, Wong CY, Ma H, Tse RT, Cheng CK, Tan M, Chiu PK, Teoh JY, Wang X, Ng CF, Zhang L
期刊名稱PROTEOMICS - Clinical Applications
出版年份2024
月份3
卷號18
期次2
出版社Wiley
文章號碼2300004
國際標準期刊號1862-8346
電子國際標準期刊號1862-8354
語言英式英語
關鍵詞bladder urine, renal pelvic urine, urine proteomics, urothelial-urinary interactions
