Aberrantly expressed microRNA in BRAF mutated young adult GBM
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AbstractAlthough glioblastoma (GBM) is predominantly found in middle age to elderly patients, young adults can be affected. Our previous study indicated a significant fraction of young adult GBM exhibited BRAF V600E mutation and they are distinct from young adult GBM showing IDH or H3F3A mutations (Zhang R et al. Oncotarget 2015). MicroRNAs (miRNAs) is a class of evolutionary conserved, short non-coding RNAs often negatively regulated expression of target genes. Studies have showed that miRNAs participate in the initiation and progression of human cancers. This study aims to identify aberrantly expressed miRNAs of BRAF mutated GBM in young adults. We first demonstrated that the miRNA profiling of matched pair of paraffin-embedded and frozen tissues was highly correlated with another. We then extended our miRNA expression profiling study to include paraffin-embedded tissues of 18 BRAF mutated GBM (age between 17 to 33) from our previous study and 5 age-matched, non-tumor brains. Unsupervised hierarchical clustering revealed that non-tumor tissues formed a distinct cluster separated from the tumors. In addition, three novel subgroups were identified among BRAF mutated GBM, with one subgroup enriched for TERT promoter mutation. Comparison to nontumor brains revealed significant upregulation of miR-21- 3p, miR-1909-5p, miR-4449, and miR-92b-3p and downregulation of miR-137, miR-129-5p, miR-338-3p, and miR-485-5p. In silico miRNA-target prediction algorithms showed these dysregulated miRNAs potentially regulated a number of important players in GBM pathogenesis, including EGFR, PDGFRA, MDM2, and TP53. Our findings suggest the involvement of miRNAs in the tumorigenesis of BRAF mutated young adult GBM.
All Author(s) ListRuiqi Zhang, Kay Ka Wai Li, Aden Ka yin Chan, Ho Keung Ng
Name of Conference2nd Asian Central Nervous System Germ Cell Tumor Conference
Start Date of Conference16/12/2016
End Date of Conference18/12/2016
Place of ConferenceTaipei
Country/Region of ConferenceTaiwan
LanguagesEnglish-United Kingdom

Last updated on 2018-18-01 at 03:07