A differentiation protocol for the generation of pancreatic beta-like cells from human embryonic stem cells
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AbstractBy virtue of their capability to replicate and regenerate, human stem-cell-derived beta-like cells could be a valuable resource for cellular therapy targeting insulin-dependent diabetes. Here, we present a protocol to generate beta-like cells from human embryonic stem cells (hESCs). We first describe steps for differentiation of beta-like cells from hESCs and CD9-negative beta-like cell enrichment through fluorescence-activated cell sorting. We then detail immunofluorescence, flow cytometry, and glucose-stimulated insulin secretion assay for characterisation of human beta-like cells. For complete details on the use and execution of this protocol, please refer to Li et al. (2020).
Acceptance Date30/06/2023
All Author(s) ListLi XS, Ma ZJ, Lui KO
Journal nameStar Protocols
Year2023
Month9
Day15
Volume Number4
Issue Number3
PublisherELSEVIER
Article number102407
ISSN2666-1667
LanguagesEnglish-United Kingdom
Keywordshuman, pancreatic, embryo, stem cell
Web of Science Subject CategoriesBiochemical Research Methods;Biochemistry & Molecular Biology