Peptide Oxidation Induced by Liquid–Solid Contact Electrification as Revealed in Liquid Microjunction-Surface Sampling Probe Mass Spectrometry
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AbstractReproducible peptide oxidation was observed using a homebuilt liquid microjunction-surface sampling probe (LMJ-SSP) platform for analyzing peptide standards. Although electrochemical oxidation and corona discharges have previously been associated with analyte oxidation in electrospray ionization (ESI) and ESI-related ambient ionization mass spectrometry (MS) methods, they were unlikely the causes for the peptide oxidation observed in the LMJ-SSP studies. A systematic investigation demonstrated that analyte oxidation was induced during the droplet drying on a solid surface through liquid–solid electrification processes. To minimize unwanted analyte oxidation, the water content in the sample solution should be decreased and the use of hydroxyl-functionalized substrates, such as glass slides, should be avoided. In addition, if water is an essential solvent component, adding an antioxidant, such as ascorbic acid, to the sample solution before droplet evaporation on the solid surface could lower the percentage of analyte oxidation. The present findings apply to all the MS methods that involve drying microliters of sample solution onto a suitable substrate in their sample preparation protocols.
Acceptance Date15/05/2022
All Author(s) ListT.-Y. Lui, Xiangfeng Chen, Simin Zhang, Danna Hu, T.-W. Dominic Chan
Journal nameAnalytical Chemistry
Year2023
Month6
Day13
Volume Number95
Issue Number23
PublisherAmerican Chemical Society
Pages9060 - 9067
ISSN0003-2700
eISSN1520-6882
LanguagesEnglish-United States
KeywordsPeptide oxidation, contact electrification, mass spectrometry

Last updated on 2024-20-08 at 00:35