The draft genome, transcriptome, and microbiome of Dermatophagoides farinae reveal a broad spectrum of dust mite allergens
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AbstractBackground
A sequenced house dust mite (HDM) genome would advance our understanding of HDM allergens, a common cause of human allergies.

Objective
We sought to produce an annotated Dermatophagoides farinae draft genome and develop a combined genomic-transcriptomic-proteomic approach for elucidation of HDM allergens.

Methods
A D farinae draft genome and transcriptome were assembled with high-throughput sequencing, accommodating microbiome sequences. The allergen gene structures were validated by means of Sanger sequencing. The mite's microbiome composition was determined, and the predominant genus was validated immunohistochemically. The allergenicity of a ubiquinol-cytochrome c reductase binding protein homologue was evaluated with immunoblotting, immunosorbent assays, and skin prick tests.

Results
The full gene structures of 20 canonical allergens and 7 noncanonical allergen homologues were produced. A novel major allergen, ubiquinol-cytochrome c reductase binding protein–like protein, was found and designated Der f 24. All 40 sera samples from patients with mite allergy had IgE antibodies against rDer f 24. Of 10 patients tested, 5 had positive skin reactions. The predominant bacterial genus among 100 identified species was Enterobacter (63.4%). An intron was found in the 13.8-kDa D farinae bacteriolytic enzyme gene, indicating that it is of HDM origin. The Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed a phototransduction pathway in D farinae, as well as thiamine and amino acid synthesis pathways, which is suggestive of an endosymbiotic relationship between D farinae and its microbiome.

Conclusion
An HDM genome draft produced from genomic, transcriptomic, and proteomic experiments revealed allergen genes and a diverse endosymbiotic microbiome, providing a tool for further identification and characterization of HDM allergens and development of diagnostics and immunotherapeutic vaccines.
All Author(s) ListCHAN Ting-Fung, JI Kun-Mei, YIM Aldrin Kay-Yuen, LIU Xiao-Yu, ZHOU Jun-Wei, LI Rui-Qi, YANG Kevin Yi, LI Jing, LI Meng, LAW Patrick Tik-Wan, WU Yu-Lan, CAI Ze-Lang, QIN Hao, BAO Ying, LEUNG Ross Ka-Kit, NG Patrick Kwok-Shing, ZOU Ju, ZHONG Xiao-Jun, RAN Pi-Xin, ZHONG Nan-Shan, LIU Zhi-Gang, TSUI Kwok Wing Stephen
Journal nameJournal of Allergy and Clinical Immunology
Year2015
Month2
Volume Number135
Issue Number2
PublisherMOSBY-ELSEVIER
Pages539 - 548
ISSN0091-6749
eISSN1097-6825
LanguagesEnglish-United Kingdom
KeywordsHouse dust mite,allergen,genome,microbiome,transcriptome,proteome,ubiquinol-cytochrome c reductase binding protein,Der f 24,Enterobacter species
Web of Science Subject CategoriesAllergy;Immunology;Allergy;Immunology

Last updated on 2020-04-08 at 02:52