MicroRNA-144 Inhibits Bone Formation in Distraction Osteogenesis through Targeting Connexin 43
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AbstractDistraction osteogenesis (DO) is a unique technique to promote bone formation in clinical practice. However, the underlying mechanism of DO is still unrevealed. Recently, microRNAs have been reported to play important roles in regulating osteogenesis. In this study, we aimed to confirm the hypothesis that some special microRNAs could regulate the bone formation during the process of DO. After successfully established the DO model of rats, a microRNA microarray and contralateral side. As the most down-reulated microRNA in the microarray result, miR-144 was also found down-regulated during osteogenic induction in mesenchymal stem cells of rats (rBMSCs). Besides, overexpression of miR-144 in rBMSC could inhibit osteogenesis by down-regulation of alkaline phosphatase, RUNX2 and BMP2, while inhibition of miR-144 would promote the osteogenesis by elevation of these osteogenic markers. Furthermore, luciferase reporter assay confirmed that connexin 43 was the direct target gene of muR-144. Finally, miR-144 inhibition rBMSCs were successfully constructed by lentivirus transfection and locally injected into the distraction gap in the DO animal. The results indicated that miR-144 inhibition therapy could promote mineralisation in DO process in vivo. In conclusion, microRNA-144 was found to regulate osteogenesis during the process of DO and inhibition of miR-144 resulted in acceleration of mineralisation found to regulate osteogenesis during the process of DO and inhibition of miR-144 resulted in acceleration of mineralisation of DO, which not only gives clues to the underlying merchanism of DO but also provides potential therapeutic targets clinically.
Acceptance Date01/09/2016
All Author(s) ListYX Sun, J Xu, LL Xu, TY Wu, B Wang, JF Zhang
Name of ConferenceHKOA 36th annual conference
Start Date of Conference05/11/2016
End Date of Conference06/11/2016
Place of ConferenceHK
Country/Region of ConferenceHong Kong
Year2016
LanguagesEnglish-United States

Last updated on 2018-18-01 at 08:21