Cerebrospinal Fluid Metabolomics After Natural Product Treatment in an Experimental Model of Cerebral Ischemia
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摘要Cerebrospinal fluid (CSF) is an important biofluid for diagnosis of and research on neurological diseases. However, in-depth metabolomic profiling of CSF remains an analytical challenge due to the small volume of samples, particularly in small animal models. In this work, we report the application of a high-performance chemical isotope labeling (CIL) liquid chromatography-mass spectrometry (LC-MS) workflow for CSF metabolomics in Gastrodia elata and Uncaria rhynchophylla water extract (GUW)-treated experimental cerebral ischemia model of rat. The GUW is a commonly used Traditional Chinese Medicine (TCM) for hypertension and brain disease. This study investigated the amine-and phenol-containing biomarkers in the CSF metabolome. After GUW treatment for 7 days, the neurological deficit score was significantly improved with infarct volume reduction, while the integrity of brain histological structure was preserved. Over 1957 metabolites were quantified in CSF by dansylation LC-MS. The analysis of this comprehensive list of metabolites suggests that metabolites associated with oxidative stress, inflammatory response, and excitotoxicity change during GUW-induced alleviation of ischemic injury. This work is significant in that (1) it shows CIL LC-MS can be used for in-depth profiling of the CSF metabolome in experimental ischemic stroke and (2) identifies several potential molecular targets (that might mediate the central nervous system) and associate with pharmacodynamic effects of some frequently used TCMs.
著者Huan T, Xian JW, Leung WN, Li L, Chan CW
期刊名稱OMICS
詳細描述A Journal of Integrative Biology.
出版年份2016
月份11
卷號20
期次11
出版社MARY ANN LIEBERT, INC
頁次670 - 680
國際標準期刊號1536-2310
語言英式英語
關鍵詞cerebrospinal fluid; chemical isotope labeling; LC-MS; metabolomics; middle cerebral artery occlusion; natural product extract
Web of Science 學科類別Biotechnology & Applied Microbiology; Genetics & Heredity

上次更新時間 2020-06-07 於 02:21