Photodynamic effect of curcumin on Vibrio parahaemolyticus
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AbstractVibrio parahaemolyticus (V. parahaemolyticus) is currently a major cause of bacterial diarrhoea associated with seafood consumption. The objective of this study was to determine the inactivation effect of curcumin-mediated photodynamic action on V. parahaemolyticus. First of all, V. parahaemolyticus suspended in PBS buffer was irradiated by a visible light from a LED light source with an energy density of 3.6 J/cm(2). Colony forming units (CFU) were counted and the viability of V. parahaemolyticus cells was calculated after treatment. Singlet oxygen (O-1(2)) production after photodynamic action of curcumin was evaluated using 9,10-Anthracenediyl-bis (methylene) dimalonic acid (ADMA). Bacterial outer membrane protein was extracted and analyzed using electrophoresis SDS-PAGE. DNA and RNA of V. parahaemolyticus were also extracted and analyzed using agarose gel electrophoresis after photodynamic treatment. Finally, the efficacy of photodynamic action of curcumin was preliminarily evaluated in the decontamination of V. parahaemolyticus in oyster. Results showed that the viability of V. parahaemolyticus was significantly decreased to non-detectable levels over 6.5-log reductions with the curcumin concentration of 10 and 20 mu M. Photodynamic action of curcumin significantly increased the singlet oxygen level with the curcumin concentration of 10 mu M. Notable damage was found to bacterial outer membrane proteins and genetic materials after photodynamic treatment. Photodynamic action of curcumin reduced the number of V. parahaemolyticus contaminating in oyster to non-detectable level. Our findings demonstrated that photodynamic action of curcumin could be a potentially good method to inactivate Vibrio parahaemolyticus contaminating in oyster. (C) 2016 Elsevier B.V. All rights reserved.
All Author(s) ListWu J, Mou HJ, Xue CH, Leung AW, Xu CS, Tang QJ
Journal namePhotodiagnosis and Photodynamic Therapy
Volume Number15
Pages34 - 39
LanguagesEnglish-United Kingdom
KeywordsCurcumin-mediated photodynamic action; Inactivation; Oyster; Singlet oxygen
Web of Science Subject CategoriesOncology

Last updated on 2020-06-07 at 02:19